Journal: Nature communications
Article Title: Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure.
doi: 10.1038/s41467-024-55295-7
Figure Lengend Snippet: Fig. 5 | SCM-198 regulates NO production through NOS3. a Detection of NO with DAF-FM DA (green) in control or SCM-198 treated AML12 cells. Scale bar, 20 µm. b NO content of the AML12 cells transfected with Adipor2 siRNA or negative control siRNA. Cells were treated with DMSO or SCM-198 for 24 h after H2O2 exposure. c NO content of the TAA/APAP-induced ALF mouse liver tissues measured at 48 hpi. Injured mice were treated with saline or SCM-198 at 24 hpi (in c, d, f, g, i–r). d NO content of WT and Adipor2-/- liver tissues, after TAA/APAP-induced ALF, measured at 48 hpi, normalized to the saline-treated group. e NO content of AML12 cells, with or without L-NAME. f Quantification of necrosis on liver sections of WT and Nos3-/-
Article Snippet: ADIPOR2 protein expression and purification His-tagged ADIPOR2 was expressed in suspension HEK293T cells by Cusabio (Wuhan, China).
Techniques: Control, Transfection, Negative Control, Saline